Advanced Cytometry Facility

The Advanced Cytomety Facility (ACF) is a collaboration of the University of Sydney, the Centenary Institute and the Bosch Institute. It is a core resource facility for Cytometry and Cell Sorting, under the aegis of the Sydney Institutes of Health and Medical Research (SIHMR) and it serves the University campus and beyond.

The Facility is supported by academic and technical expertise and underpinned by cutting-edge instrumentation. A steering committee chaired by a senior University of Sydney academic oversees the operation of this resource partnership.

The ACF is committed to the continuing development and transfer of cytometry expertise and techniques and enhancing the role of cytometry as a major modality in biological/biomedical research. We aim to take current research to the next level of discovery and maintain our position on the cutting-edge through the ongoing search for the funding to support the people and instruments that make this possible.

Instruments

	Analysers					Sorters
	Calibur	Canto	LSRII	Image stream		Aria (Blackburn)	Aria (Centenary)	Vantage
Laser Line	number of PMT's per laser line
UV - 355 nm			2
Violet - 405 nm			3				3
Blue - 488 nm	3	4	5	3		5	5	5
Red - 633 nm	1	2	3	1 (658nm)		2	3
Dye laser - tune 570-610nm								2
I70C spectrum - 355/457/488/521/588/647 nm								2
Sort Receptacles						5ml - 4ways 15ml - 2 ways Plate 6- 384 well slide	5ml - 4ways 15ml - 2 ways Plate 6- 384 well slide	5ml - 4ways 15ml - 2 ways Plate 6- 384 well slide

Procedure for accessing ACF instrumentation

1. Download and complete (i) New User/Project Form and (ii) Biosafety Form and return to facility staff (see below)
2. Arrange a meeting with an facility academic to discuss project feasibility and agree on acknowledgment/collaboration arrangements
3. Outcomes of meeting:-
   • another approach is recommended
   • hands on training is arranged
4. Experiments can proceed after training is complete and biosafety approval is granted

Forms

• Forms can filled out and saved using Acrobat Reader version 7.0.9 or 8 (or higher).
• Please return electronic versions to enquiries@cytometrynsw.org.au so we can compile data.
• Signed copies can be brought to the Project Meeting or faxed to Adrian Smith on 95656101.
• Download: ACF New User Form
• Download: ACF Biosafety Form

Introduction to Flow Cytometry Workshop - May 2008

1. Introduction to Flow Cytometry (0.4MB PDF)
2. Applications of Flow Cytometry (4.5MB PDF)
3. Flow Data & Compensation (3.8MB PDF)
4. ACF Instruments (1.5MB PDF)